California Current Ecosystem LTER

Microplastic Numerical and Mass Concentration

Title
Numerical and mass concentrations of microplastic debris collected by manta net; Numerical concentrations of Halobates sericeus adults/juveniles and eggs; zooplankton biomass concentration. Various cruises from 1972 - 2010 (completed).

Abstract
Microplastic, Halobates, and zooplankton were collected using a standard manta net tow. Each sample was sorted at 6-12x magnification under a Wild M-5 dissecting microscope, and plastic particles and H. sericeus removed for further analysis. Plastic particles were soaked in deionized water to remove salts, dried at 60°C, and stored in a desiccator. Dry mass was measured on an analytical balance. Particles were then digitally imaged with a Zooscan digital scanner (Gorsky et al. 2010). The total number of particles was measured using NIH ImageJ-based tools in the Zooprocess software, calibrated against manual measurements (Gilfillan et al. 2009, Gorsky et al. 2010). H. sericeus samples were enumerated and classified into 5 categories: juvenile, adult male, adult female, newly molted, and molted exoskeletons. H. sericeus eggs, both those attached to plastic and those that had become detached during the collection process, were also enumerated. Dry mass of zooplankton was obtained from preserved manta tow samples. After fixation in 1.8% formaldehyde for 24 months, samples were split in a Folsom splitter, filtered onto 202 µm Nitex mesh disks and rinsed with isotonic ammonium formate. Filters were dried for 24 hours at 60°C and placed in a vacuum dessicator until weighing. Filters were weighed to the nearest 0.0001 gram on the same analytical balance as the plastic samples. A 20% correction factor was applied in order to compensate for the biomass lost by preservation.

Keywords
oceans, pollution, ecosystem ecology, measurements, biomass, particles

Projects
California Current Ecosystem LTER

Creators
Goldstein, Miriam (miriam.goldstein@gmail.com)

Contact
CCE LTER Information Manager (ccelter-im@ucsd.edu)

General Methods Microplastic was collected using a standard manta net tow. (link: http://swfsc.noaa.gov/textblock.aspx?Division=FRD&ParentMenuId=213&id=1342). Each sample was sorted at 6-12x magnification under a Wild M-5 dissecting microscope, and plastic particles removed for further analysis. Plastic particles were soaked in deionized water to remove salts, dried at 60°C, and stored in a desiccator. Dry mass was measured on an analytical balance. Particles were then digitally imaged with a Zooscan digital scanner (Gorsky et al. 2010). The total number of particles was measured using NIH ImageJ-based tools in the Zooprocess software, calibrated against manual measurements (Gilfillan et al. 2009, Gorsky et al. 2010). This dataset is compiled from data collected during research cruises conducted and funded by different agencies. Please refer to the particular study metadata and accompanying documents for differences in methodologies, or contact the current provider for this dataset. Not every column applies to all studies, but are included because of the tabular format in which these data are stored. The following specifies which fields are non-applicable to which studies: STAR cruises: Event Index, Event Number, Tow Number, Halobates Abundance, Halobates Egg Abundance EX1006: Event Number, Grid Line, Grid Station SEAPLEX: Tow Number, Grid Line, Grid Station

Available Data

Type Name Description Info
MicroplasticNumericalandMassConcentration Main data table for dataset Rows: 235
Columns: 19
View / Download