California Current Ecosystem LTER

Picoplankton and Bacteria Biomass (CalCOFI Cruise)

Title
Picophytoplankton and bacteria total carbon estimates from cell counts analyzed with flow cytometry (FCM) from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2019 (ongoing).

Abstract
Picophytoplankton populations and non-pigmented prokaryotes are sampled within the California Current Ecosystem (CCE) for abundances from 3 to 8 depths at CalCOFI stations. Seawater is collected from Niskin bottles and cells are fixed in the field aboard the survey cruises (since 2004, ongoing) with paraformaldehyde, and stained with a DNA-specific dye back in the laboratory. The cells are enumerated by an Altra flow cytometer (with a syringe pump for volumetric sample delivery) simultaniously with argon ion lasers, to distinguish three major populations of photoautotrophs (Prochlorococcus, Synechococcus, and pico-eukaryotes) and the assemblage of heterotrophic prokaryotes collectively referred to as H-Bact. FCM abundance estimates for each are converted to carbon biomass equivalents using mixed-layer estimates.

Keywords
marine, ecology, biomass, phytoplankton, bacteria, flow cytometry

LTER Data System Record
http://dx.doi.org/10.6073/pasta/994126a7ba3d90250cf371d92b134538
Projects
California Current Ecosystem LTER

Creators
Landry, Michael (mlandry@ucsd.edu)

Contact
CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table PicoplanktonandBacteriaBiomass
Main data table for dataset
Rows: 12792
Columns: 14
View / Download

Methods


Biomass Estimates
Data revised and updated Nov. 2015, with biomass estimates using constant factors of 11, 32 and 100 fgC/cell for Hbact, Pro and Syn, respectively.