California Current Ecosystem LTER

Chlorophyll (P0904, Student Cruise)

Title
Chlorophyll and phaeopigments measured from discrete bottle samples from CCE LTER P0904 student cruise in the California Current System, determined by extraction and bench fluorometry, April 2009.

Abstract
Discrete bottle samples taken from various depths in the California Current System aboard CCE LTER P0904 student cruise. Samples were frozen aboard the ship and taken back to the lab for extraction and analysis. Samples are filtered (known volumes) onto GF/F filters. The filters are placed into culture tubes containing 90% acetone, and the fluorescence of the samples is read on a fluorometer after 24 to 48 hours. The samples are then acidified to degrade the chlorophyll to phaeopigments (non-photosynthetic pigments) and a second reading is taken. The readings prior to and after acidification are used to calculate concentrations of both chlorophyll a and phaeopigments (i.e. phaeophytin).

Keywords
marine, measurements, chlorophyll, fluorescence, phaeopigments, CTD

LTER Data System Record
http://dx.doi.org/10.6073/pasta/75eab59ad7c9fb7a2d1bf8b74e77178e
Projects
California Current Ecosystem LTER

Creators
Stukel, Mike (mstukel@fsu.edu)

Contact
CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table Chlorophyll
Water column chlorophyll measurements
Rows: 153
Columns: 6
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Methods


General Methods
Chlorophyll determined using chlorophyll extraction methodology. Samples concentrated on GF/F filters.