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Title: Chl a from discrete bottles samples from the Cal Poly Pier, 2017-present.
Abstract: Water for chlorophyll a is sampled from three depths (~1, 4 and 9 m) using niskin bottles attached to a CTD rosette.The concentration of chlorophyll a is determined by filtering water though filters of differing pore sizes including a GFF (nominal pore size 0.7 µm) and 3 µm filter. These samples are extracted in 90% acetone and analyzed fluorometrically using a Triology Fluorometer.

Keywords: chlorophyll, concentration, CTD, fluorescence, oceans, Cal Poly, pier, time series, Avila
LTER Data System Record: http://dx.doi.org/10.6073/pasta/c6dec375367d080de090e9fdc47791ef

Creators: Pasulka, Alexis (apasulka@calpoly.edu)
Contact: CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table

Chlorophyll
Main data table

Rows: 184
Columns: 13

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Methods

General Methods

Chl a samples were processed by filtering a known volume of seawater (0.1L) thru 2 different filter pore sizes (Whatman GF/F and 3µm nucleopore filter) in triplicate. Filters were extracted in 4.5 mls of 90% acetone and stored in the dark -20degC for approximately 24 hours. Samples were thawed in the dark until reaching room temperature and then analyzed on a Triology Fluorometer. Chlorophyll a was calculated from fluorescence using the acidification method (Strickland & Parsons 1972). Fluorometer calibrations using a chlorophyll a standard (Sigma Cat#C6144) were acquired from the CalCOFI group.

California Current Ecosystem LTER

Cal Poly Pier Chlorophyll

Data

Methods