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Title: Advanced Laser Fluorescence (ALF) measurements of chlorophyll-a (Chl), phycoerythrin (PE), chromophoric dissolved organic matter (CDOM), and variable fluorescence (Fv/Fm) from CCE process cruises in the California Current System, 2006 - 2012.
Abstract: The Advanced Laser Fluorometry (ALF) provides spectral deconvolution (SDC) analysis of the laser-stimulated emission (LSE) excited at 405 or 532/510 nm for assessment of chlorophyll a, phycoerythrin, and chromophoric dissolved organic matter. Three spectral types of phycoerythrin are discriminated for characterization of cyanobacteria and cryptophytes in mixed phototrophic populations. The SDC analysis is integrated with measurements of variable fluorescence, Fv/Fm, corrected for the SDC-retrieved background fluorescence, BNC, for improved photophysiological assessments of phytoplankton photochemical efficiency. Fluorescence measurements are normalized to the water Raman scattering to improve the accuracy of measurements. The ALF measurements of discrete water samples from various depths were made using several ALF instrument modifications (ALF-1, ALF-T, Aquatic Laser Fluorescence Analyzer (ALFA) , or Custom Laser Analytical Spectroscopic System (CLASS)) during CCE process cruises.

Keywords: biomass, chlorophyll, fluorescence, marine, physiology, phytoplankton, Fv/Fm, CDOM, colored dissolved organic carbon, phycobiliproteins, phycoerythrin, physiological state, pump-during-Probe
LTER Data System Record: http://dx.doi.org/10.6073/pasta/4e3776445b3d255783bd24470008b0bb

Projects: California Current Ecosystem LTER
Creators: Chekalyuk, Alexander (chekaluk@ldeo.columbia.edu)
Contact: CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table

alf sample data
primary data table for dataset

Rows: 1060
Columns: 23

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Methods

General Methods

Advanced Laser Fluorescence (ALF) measurements of discrete water samples from various depths are made using ALF instruments (ALF (Chekalyuk and Hafez, 2008), ALF-T (Chekalyuk and Hafez, 2013), Aquatic Laser Fluorescence Analyzer (ALFA; Chekalyuk et al. 2014), and Custom Laser Analytical Spectroscopic System (CLASS)) during CCE process cruises. The instruments use excitation at 405 and 510 (ALF-T, CLASS) or 532 (ALF, ALFA) nm to measures the fluorescence of chlorophyll-a (Chl) and chromophoric dissolved organic matter (CDOM) used for assessment of Chl concentration and CDOM content. Three types of phycoerythrin are spectrally discriminated for characterization of cyanobacteria and cryptophytes in phytoplankton populations. Variable fluorescence (Fv/Fm) is measured to assess phytoplankton photochemical efficiency. Fluorescence measurements are normalized to the water’s Raman scattering to improve the accuracy of measurements. The ALF fluorescence measurements of discrete water samples can be used for assessment of Chl concentration, phytoplankton photophysiology, community structure, total autotrophic carbon biomass, Synechococcus carbon biomass, CDOM content, and other characteristics (e.g., Chekalyuk et al., 2012).

References

A. M. Chekalyuk and M. Hafez, “Advanced laser fluorometry of natural aquatic environments,” Limnol. Oceanogr. Methods 6, 591–609 (2008).

A. M. Chekalyuk and M. Hafez, "Next generation Advanced Laser Fluorometry (ALF) for characterization of natural aquatic environments: new instruments," Opt. Express 21, 14181-14201 (2013).

A.M. Chekalyuk, M.R. Landry, R. Goericke, A.G. Taylor, and M. Hafez. J. "Laser fluorescence analysis of phytoplankton across a frontal zone in the California Current ecosystem," Plankton Res., 34(9), 761-777 (2012)

A.M. Chekalyuk, A. Barnard, A. Quigg, M. Hafez, and Y. Zhao. "The Aquatic Laser Fluorescence Analyzer: field evaluation in the northern Gulf of Mexico," Optics Express 22, 21641-21656 (2014).

California Current Ecosystem LTER

ALF Active Fluorescence Measurements – Sample Data (Process Cruise)

Data

Methods

References