California Current Ecosystem LTER

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High Performance Liquid Chromatography Pigments (Process Cruise)

Title
High Performance Liquid Chromatography (HPLC) pigment analysis from rosette bottle samples at various depths from CCE LTER process cruises in the California Current System, 2006 to 2017.

Abstract
High Performance Liquid Chromatography (HPLC) samples are collected from rosette bottles (from three to eight different depths in the photic zone) at stations located within the CCE region on Process cruises (since 2006, ongoing). The HPLC method is used to measure concentrations of chlorophylls and carotenoids in samples of particulate matter, which includes filtering and freezing the filter while at sea. The taxon-specific phyto-pigments are extracted back onshore. Concentrations of chlorophyll a are used as a proxy for phytoplankton biomass and concentrations of other taxon-specific pigments are used to determine contributions of phytoplankton taxa to total phytoplankton biomass.
Keywords
marine, measurements, chlorophyll, phytoplankton, CTD, concentration, Primary Production, HPLC

LTER Data System Record
http://dx.doi.org/10.6073/pasta/831e099fb086954d3d73638d33d3dd05
Projects
California Current Ecosystem LTER

Creators
Goericke, Ralf (rgoericke@ucsd.edu)

Contact
CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table HighPerformanceLiquidChromatographyPigments
Main data table for dataset 		Rows: 464
Columns: 28 		View / Download

Methods

General Methods
Samples will be concentrated on GF/F or Nuclepore filters (total or sized-fractioned samples) and stored in liquid N2. Pigments are extracted in acetone and analyzed by reverse-phase HPLC for major chlorophylls and taxon-specific carotenoids (Goericke & Repeta 1993). Chlorophyll contributions by the major phytoplankton groups are calculated from class-specific accessory pigments according to the primary and ancillary ratio approach of Goericke & Montoya (1998). In combination with microscopic analyses, group-specific carbon ratios (e.g., Cdiatom:fucoxanthin) will be determined to assess pigment ratio variability as a function of nutrient concentration and light condition, and to evaluate pigment acclimation effects in experimental incubations for growth and grazing rates.

Frequency
Survey Grid

Protocols

Taxon-specific phyto-pigments
http://cce.lternet.edu/data/methods-manual/augmented-cruises/taxon-specific-phyto-pigments

References

Goericke, R., The Structure of Marine Phytoplankton Communities — Patterns, Rules and Mechanisms. CalCOFI Reports, 52, 182-197, 2011. CCE LTER Contribution #0213.

Peloquin, J., C. Swan, N. Gruber, M. Vogt , H. Claustre, J. Ras, J. Uitz, R. Barlow, M. Behrenfeld, R. Bidigare, H. Dierssen, G. Ditullio, E. Fernandez, C. Gallienne, S. Gibb, R. Goericke, L. Harding, E. Head, P. Holligan, S. Hooker, D. Karl, M. Landry, R. Letelier, C. A. Llewellyn, M. Lomas, M. Lucas, A. Mannino, J.-C. Marty, B. G. Mitchell, F. Muller-Karger, N. Nelson, C. O'Brien, B. Prezelin, D. Repeta, W. O. Jr. Smith, D. Smythe-Wright, R. Stumpf, A. Subramaniam, K. Suzuki, C. Trees, M. Vernet, N. Wasmund, and S. Wright, The MAREDAT global database of high performance liquid chromatography marine pigment measurements. Earth System Science Data, 5, 109-123, 2013. DOI 10.5194/essd-5-109-2013. CCE LTER Contribution #0244.

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