California Current Ecosystem LTER

Picoplankton and Bacteria Biomass (Process Cruise)

Title
Picophytoplankton and bacteria total carbon estimates from cell counts analyzed with flow cytometry (FCM) from CCE LTER process cruises in the California Current region, 2006 - 2017.

Abstract
Picophytoplankton populations and non-pigmented prokaryotes are sampled within the California Current Ecosystem (CCE) for abundances from various depths. Seawater is collected from Niskin bottles and cells are fixed in the field aboard the survey cruises (since 2004, ongoing) with paraformaldehyde, and stained with a DNA-specific dye back in the laboratory. The cells are enumerated by an Altra flow cytometer (with a syringe pump for volumetric sample delivery) simultaniously with argon ion lasers, to distinguish three major populations of photoautotrophs (Prochlorococcus, Synechococcus, and pico-eukaryotes) and the assemblage of heterotrophic prokaryotes collectively referred to as H-Bact. FCM abundance estimates for each are converted to carbon biomass equivalents using mixed-layer estimates.

Keywords
habitats, marine, ecology, measurements, biomass, debris flows, flow cytometry

LTER Data System Record
http://dx.doi.org/10.6073/pasta/4d6638460b8a3e8c5ccb95cde821b3ae
Projects
California Current Ecosystem LTER

Creators
Landry, Michael (mlandry@ucsd.edu)

Contact
CCE LTER Information Manager (ccelter.im@gmail.com)

Data

table PicoplanktonandBacteriaBiomass
Main data table for dataset
Rows: 1876
Columns: 13
View / Download

Methods


Biomass Estimates
Data revised and updated Nov. 2015, with biomass estimates using constant factors of 11, 32 and 100 fgC/cell for Hbact, Pro and Syn, respectively.